3’-Dephosphocoenzyme A, Sodium Salt
CAS No.: N/A
Synonym(s): 3’-Dephosphocoenzyme A, Dephospho-CoA, depCoAAvailability: 6-8 weeks
3'-Dephosphocoenzyme A (depCoA) is the immediate precursor to coenzyme A and the final intermediate in its universal biosynthetic pathway.¹ ² ³ It is formed by PPAT (4′-phosphopantetheine adenylyltransferase), which transfers an adenylyl group from ATP to 4′-phosphopantetheine. The final step of CoA synthesis is catalyzed by dephospho-CoA kinase (DPCK), which phosphorylates depCoA to produce CoA.¹ ² ³ ⁴ Structural studies show that DPCK contains core, CoA-binding, and LID domains that undergo conformational changes upon substrate binding to shield the active site.⁴
Applications
3'-Dephosphocoenzyme A is used in drug discovery as part of antimalarial research targeting DPCK in Plasmodium falciparum, where selective inhibitors are screened to avoid affecting human orthologues.¹ It is also widely used in RNA biology as a transcription initiator for generating CoA-RNA conjugates, enabling studies of RNA–small molecule interactions and RNA-linked metabolic regulation.² In biotechnology, depCoA supports synthesis of thiol-functionalized RNA for purification, surface immobilization, and therapeutic conjugation. Additionally, 3'-Dephosphocoenzyme A is used in enzymatic synthesis platforms and in structural studies of kinase mechanisms in bacteria such as E. coli and Legionella pneumophila.² ³ ⁴
Other available forms
