Phenylacetyl Coenzyme A, Sodium salt
CAS No.: N/A
Synonym(s): Phenylacetyl Coenzyme A, Phenylacetyl-CoA sodium, Phenylacetyl-CoA Na salt
Phenylacetyl coenzyme A (PhAc-CoA) is an aromatic thioester and key intermediate in the phenylacetyl-CoA catabolon, a bacterial pathway for degradation of diverse aromatic compounds including styrene, ethylbenzene, phenylethylamine, and related substrates.¹ ² It is formed from phenylacetic acid by phenylacetyl-CoA ligase (PaaK) and functions as a regulatory “true inducer” by binding repressors such as PaaX and PaaR, thereby controlling expression of aromatic catabolic genes in bacteria including Pseudomonas putida and Thermus thermophilus.¹ ²
Applications
Phenylacetyl-CoA is used in biotechnology to improve benzylpenicillin production in Penicillium chrysogenum through expression of phenylacetyl-CoA ligase (PaaK). It is also applied in the production of biodegradable aromatic plastics, including poly(3-hydroxyphenylalkanoates) (PHPhAs), from bacterial metabolic systems. In research, PhAc-CoA is used to study enzyme kinetics of “hotdog” fold thioesterases and to analyze protein–ligand interactions using BIAcore biosensor assays and isothermal titration calorimetry (ITC).¹ Additionally, Phenylacetyl-CoA serves as a biochemical tool for characterizing regulatory mechanisms in aromatic catabolic pathways.¹ ²
Other available forms
