Methylmalonyl Coenzyme A, Free acid
CAS No.: 1264-45-5
Synonym(s): Methylmalonyl Coenzyme A, Isosuccinyl coenzyme A, Methylmalonyl-CoAAvailability: 6-8 weeks
Methylmalonyl-CoA is a central metabolic intermediate derived from the catabolism of amino acids (valine, isoleucine, methionine, threonine), odd-chain fatty acids, and cholesterol.¹ ² It is converted into succinyl-CoA by the vitamin B12-dependent enzyme methylmalonyl-CoA mutase (MCM), enabling entry into the TCA cycle.¹ ³ In clinical medicine, deficiencies in MCM or vitamin B12 metabolism cause methylmalonic acidemia (MMA), characterized by accumulation of methylmalonic acid and symptoms such as metabolic acidosis and developmental disorders.¹
Applications
Methylmalonyl-CoA is used in clinical diagnostics to assess MCM activity, with UPLC–MS/MS assays enabling precise measurement in peripheral lymphocytes.¹ In biotechnology, (2S)-methylmalonyl-CoA is a key extender unit for polyketide biosynthesis, including the erythromycin precursor 6-deoxyerythronolide B (6-dEB). Engineered E. coli strains produce this metabolite via mutase–epimerase pathways or metabolic optimization (e.g., ygfH deletion) to increase product yield.² ⁴ Additionally, organisms such as Corynebacterium glutamicum accumulate methylmalonate during propionate metabolism, influencing growth and metabolic regulation.³
Other available forms
