Octanoyl Coenzyme A, Lithium salt
CAS No.: 324518-20-9
Synonym(s): Octanoyl Coenzyme A, Capryloyl coenzyme A lithium salt, Capryloyl coenzyme A lithium salt hydrate
Octanoyl Coenzyme A (Octanoyl-CoA) is a medium-chain thioester intermediate used in microbial biosynthesis of high-value esters for flavor, fragrance, and biofuel applications.¹ ² In Saccharomyces cerevisiae, the enzyme Eht1 shows highest activity with C8 substrates and functions as both an acyltransferase and thioesterase, which may help regulate intracellular CoA levels during fermentation.² In Escherichia coli, octanoyl-CoA is a β-oxidation intermediate whose availability can be increased by deleting fadE to prevent degradation.¹
Applications
Octanoyl-CoA is used as a substrate in microbial production of esters such as ethyl octanoate and butyl octanoate.¹ ² It supports biochemical studies of alcohol acyltransferases, including Eht1, their acyltransferase and thioesterase activities, and their substrate specificity for medium-chain acyl-CoAs.² It is also used in metabolic engineering to optimize ester yields by limiting β-oxidation and improving pathway flux, including in engineered hosts such as E. coli.¹ Octanoyl-CoA is a substrate for medium-chain acyl-CoA dehydrogenase (MCAD) and is relevant to enzyme activity assays and investigations of MCAD deficiency.³ It also serves as the acyl donor for ghrelin octanoylation by ghrelin O-acyltransferase (GOAT), linking it to studies of appetite-regulating hormone modification.⁴ Octanoyl-CoA might be used as an HPLC or LC-MS reference standard.⁵
Other available forms
